Effects of Temperature on Enzyme Activity

Problem: To what extent does temperature affect enzyme activity rate?

Research: Use page 52 in your text book to find facts about catalase and hydrogen peroxide.







Materials: hydrogen peroxide, 50 ml beaker, distilled water, filter paper disks, forceps, stopwatch, ice, 500ml beaker, and hotplate


  1. Cut a small slice of potato to take to your lab table.
  2. Pour 10 ml of hydrogen peroxide into a 50 ml beaker.
  3. Pour 40 ml of distilled water into the beaker to make a total liquid volume of 50 ml.
  4. Take the temperature of the water / peroxide mixture in degrees celsius and record it in the data table
  5. Dip a disk of filter paper (cut with a hole punch) into the freshly cut potato's wet liquid until it is soaked completely.
  6. When the timer says go, force the filter paper to the bottom of the 50 ml beaker with peroxide and water.
  7. Record the number of seconds it takes for the paper to reach the top.
  8. Repeat steps 5 through 7 at temperature intervals of 2 degrees while either heating the 50 ml beaker in a water bath (500 ml beaker with 2 cm of water) on a hotplate (turned on low), or while cooling it in a water bath (500 ml beaker with 2cm water and ice).
  9. Record and graph the data
  10. Make your conclusion

Data Table an Graph:

Temperature (degrees C) Seconds to reach the surface
to Reach

Temperature degrees C


1) What did YOUR data show you about the effect of temperature on enzyme activity rate?

2) What went well with your experiment?

3) What were possible extraneous variables in your techniques?

4) What new questions could be future problems to study now that you know the effects of temperature on enzymes?

5) What did you learn from this lab?